Once you have generated and approved your Jacquard content, you can navigate to AJO to set up your Campaign as you normally would, including configuring the audience and initial content.
The feature you are going to utilise is Content experiment, from the Action section of the Campaign creation process:
After pressing Create experiment, you need to choose a success metric.
Then, add treatments to create versions for each of your Jacquard content variants, up to 9 (i.e. treatment I) in total. Ensure you distribute the audience evenly.
Now that these are added, return to Edit content:
Update the subject line (or body copy) of each treatment with a different variant from Jacquard.
After reviewing your content, you can Activate the campaign.